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Abstract

Sirih merah (Piper crocatum Ruiz & Pav.) merupakan tanaman yang tumbuh di Indonesia yang memiliki manfaat sebagai tanaman herbal. Manfaat daun sebagai tanaman herbal karena memiliki senyawa aktif yang dapat digunakan sebagai senyawa dalam metabolisme di dalam tubuh tumbuhan. Senyawa aktif tersebut
dapat dianalisis dengan berbagai cara. Tujuan dari penelitian ini adalah untuk mengetahui kandungan total flavonoid, total fenol dan aktivitas antioksidan (IC50) ekstrak etanol daun sirih merah. Ektraksi daun sirih merah dilakukan dengan metode maserasi menggunakan etanol 50 dan 70%. Analisis total flavonoid dan kandungan total fenol dilakukan dengan metode Folin ciocalteau, sedangkan aktivitas antioksidan ditentukan menggunakan metode DPPH. Hasil penelitian menunjukan bahwa senyawa total flavonoid dari ekstrak etanol
50% berkisar 155,27 mg QE/g, sedangkan kandungan total fenol sebesar 142,56 mg GAE/g. Pada ekstrak etanol 70% total flavonoid sebesar 168,33 mg QE/g dan total fenol sebesar 157,61 Mg GAE/g. Adapun rata–rata aktivitas antioksidan (IC50) pada ekstrak etanol 50% adalah 132,52 ppm dan pada ekstrak etanol 70%
sebesar 129,11 ppm.

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How to Cite
, , & . (2018). Karakteristik (Total Flavonoid, Total Fenol, Aktivitas Antioksidan) Ekstrak Serbuk Daun Sirih Merah (Piper crocatum Ruiz & Pav.). Food Science and Technology Journal (Foodscitech), 1(2), 26-34. https://doi.org/10.25139/fst.v1i2.1355

References

  1. Alfaribi, M., Bintang, M., Suryani and Safithri. 2010. The Comparative Ability of Antioxidant Activity of Piper crocatum in Inhibiting Fatty Acid Oxidation and Free Radical Scavenging. HAYATI Journal of Biosciences, 17(4), 201-204.
  2. Anugrahwati, M., Purwaningsih, T., Rustina, Manggalarini, J.A., Alnavis, N. B., Wulandari, D. N., & Pranowo, H.D., 2016. Extraction of Ethanolic Extract of Red Betel Leaves and Its Cytotoxicity Test on HeLa Cells, Procedia Engineering, vol.148, pp.1402-1407
  3. Arambewela, L.S.R. 2005. Antidiabetic Activities Of Agneous And Ethanolic Extracts Of Piper Betle Leaves In Rats. J of Ethno Pharmacology 102: 239-245.
  4. Atanassova, M., Georgieva S., and Ivancheva K. 2011. Total Phenolic And Total Flavonoid Contents, Antioxidant Capacity And Biological Contaminants In Medicinal Herbs. Journal of the University of Chemical Technology and Metallurgy, 46, 1, 2011, 81-88.
  5. Chang, H.Y., HO, Y.L. Sheu, M.J., Lin, Y.H. Tseng., M.C. Wu., S.H. Huang., G.J. and Chang, Y.S. 2007. Antioxidant and Free Radical Scavenging Activities of Phellinus merrillii Extracts. Botanical Studies 48: 407-417.
  6. Craft, B.D., Kerrihard, A.L., Amarowicz, R., & Pegg, R.B., 2012. Antioxidant and in the in Vitro Methods Used for Their Assessment, Comprehensive Reviews in Food Science and Food Society, vol.12, 2012
  7. Cragg, G.M., and Newman, D.J. 2005. Plants as a source of anti-cancer agents. J.Etnopharmacol, 100:72-79
  8. Deore, S.L. et al. 2009. In vitro antioxidant activity and phenolic content of Croton caudatum. International Journal of Chemical Technology Research, 1(2), P. 174-176.
  9. Emrizal., Armon, F., Riska, Y., Kamal, R., Nola, R.I, Adriani S., Reni, Y., Farediah A., Hasnah M.S., And Dayar, A. 2014. Cytotoxic Activities of Fractions and Two Isolated Compounds from Sirih Merah (Indonesian red betel), Piper crocatum Ruiz & Pav. Procedia Chemistry 13 ( 2014 ) 79 – 84.
  10. Kim, D.O., Lee, K.W., Lee, H.J and Lee, C.Y. 2002. Vitamin C Equivalent Antioxidant Capacity (VCEAC) of Phenolic Phytochemicals. Journal of Agricultural and Food Chemistry 50: 3713 -3717.
  11. Lee, K. W., Kim, Y. J., Lee, H. J And Lee, C. Y. 2003. Cocoa Has More Phenolic Phytochemical And Higher Antioxidant Than Teas And Red Wine. J. Agric. Food Chem, 51(25): 249-252.
  12. Li, C., Du, H., Wang, L., Ahu, Q., Zheng, Y., Xu, Y., Zhang, J., Zhang, J., Yang, R And Ge, Y. 2009. Flavonoid composition and antioxidant activity of tree peony (Paeonia Section Moutan) yellow flowers”. Journal of Agricultural and Food Chemistry, 57, hal 8496–8503.
  13. Lin, H.Y., Kuo, Y.H., Lin, Y.L., & Chiang, W., 2009. Antioxidative Effect and Active Components from Leaves of Lotus (Nelumbo nucifera), Journal of Agricultural and Food Chemistry, vol.57, no.15, pp. 6623–6629
  14. Lister, I.N.E., Rizka D.V., Ali, N.N., Rahmiana, Z., Yunazar, M., and Edison, M. 2014. Antimicrobial Activities Of Methanol Extract Of Sirih Merah (Piper Crocatum L.) Lleaf. Journal Of Chemical And Pharmaceutical Research, 2014, 6(12):650-654
  15. Manthey, J. A. 2004. Fractionation of orange peel phenols in ultrafiltered molasses and mass balance studies of their antioxidant levels. Journal of Agricultural and Food Chemistry 52: 7586-7592.
  16. Maulidha, N., Fridayanti, A., and Masruhim, M.A. 2015. Uji Aktivitas Antioksidan Ekstrak Daun Sirih Hitam (Pipersp.) Terhadap DPPH (1,1-Diphenil-2 Pichryl Hydrazil). Jurnal Sains dan Kesehatan. 2015. Vol 1. No 1. p-ISSN: 2303-0267, e-ISSN: 2407-6082
  17. Molyneux, P. 2004. The Use Of The Stable Free Radikal Diphenylpicrylhydrazyl (DPPH) For Estimating Antioxidant Activity. Journal Science Of Technology, 26 (2):211-219.
  18. Nakiboglu, M.U., R.O. Kayali., H.A. And Tarhan, L. 2007. Antioxidant capacities of endemic sideritis sipylea and origanum sipyleum from turkey. Food Chemistry, 104, hal 630–635.
  19. Nisa, G.K., Wahyunanto, A.N., and Yusuf, H. 2014. Extraction Of Red Betel Leaf (Piper Crocatum) Methods Microwave Assisted Extraction (MAE). Jurnal Bioproses Komoditas Tropis. Vol. 2 No. 1.
  20. Nouri, L., Nafchi, A.M., And Karim, A.A. 2014. Phytochemical, Antioxidant, Antibacterial And α-Amylase Inhibitory Properties of Different Extract From Betel Leaves. Industrial Crops And Products 62 (2014) 47-52.
  21. Prameswari, O.M, dan Widjanarko, S.B. 2014. The Effect of Water Extract of Pandan Wangi Leaf to Decrease Blood Glucose Levels and Pancreas Histopathology at Diabetes Mellitus Rats. Jurnal Pangan dan Agroindustri Vol.2 No.2 p.16-27
  22. Reveny, J. 2011. Daya Antimikroba Ekstrak Dan Fraksi Daun Sirih Merah (Piper Betle Linn.). Jurnal ILMU DASAR, Vol. 12 No. 1, Januari 2011: 6-12.
  23. Schmidt , S., M. Zietz., M. Schreiner., S. Rohn., L.W. Kroh., A. Krumbein. 2009. Genotypic and Climatic Influences on the Concentration and Composition of Flavonoids in Kale (Brassica oleracea var. sabellica). Food Chemistry.119: 1293–1299.
  24. Serlahwaty, D., Sugiastuti, S., dan Ningrum, S.C. 2011. Aktivitas Antioksidan Ekstrak Air dan Etanol Daun Sirih Hijau (Piper betel L.) dan Sirih Merah (Piper cf. fragile Benth.) dengan Peredaman Radikal Bebas [skripsi]. Jakarta. Fakultas Farmasi. Universitas Pancasila, Jakarta.
  25. Sharma, O.P., and Bhat, T.J. 2009. DPPH Antioxidant Assay Revisited. Food Chemistry 113 (2009) 1202–1205.
  26. Swapna, N.L., K. Ammani., And H. K. R. Prasad Saripalli. 2012. Antioxidant activity of Mokkathotapapada leaves of Piper betel L. Cv. Kapoori.Free Radicals and Antioxidants Vol.2 / Issue 4 / Oct–Dec, 2012.
  27. DOI: 10.5530/ax.2012.4.12
  28. Tiwari, P., Kumar, B., Kaur G., Kaur, H., and Kaur, M. 2011. Phytochemical Screening and Extraction : A review. J Int Pharm Sci 1 : 98-106
  29. Werdhany, W., Anthoni, M., dan Setyorini, W. 2008. Sirih Merah. Primatani Kotamadya Yogyakarta. Balai Pengkajian Teknologi Pertanian Yogyakarta.