Reader Comments

Damage in PubMed ID: live cells, thus defining HMGB1 as a component of

by Terese Lara (2020-09-02)

Damage in live cells, Nutlin 3a thus defining HMGB1 as a component of an early DNA damage response [51]. A similar function has been attributed to HMGB2 [52]. These authors hypothesized that HMGB1/2 proteins act as a sensor of DNA modification, and their interaction with chemically altered DNA changes the chromatin structure, thus inducing DNA damage responses. PubMed ID: Finally, a cluster related to tRNA metabolic processes was also significant (TRNT1, METTL1, ADAT2, NSUN2, IARS, GARS, EPRS and MARS). Aminoacyl-tRNA synthetases perform an essential function in protein synthesis by catalyzing the esterification of an amino acid to its cognate tRNA (IARS, GARS, EPRS and MARS). Considering all together, we speculate that in the SF, vitamin E exerts antiinflammatory effect and stress protection by increasing heat shock protein expression. Vitamin E reduces stress response probably as results of reduced reactive oxygen species levels. In addition animals supplemented with VE might have inhibited cholesterol oxidation in SF and enhanced sterol, steroid and cholesterol biosynthesisGonz ez-Calvo et al. BMC Genomics (2017) 18:Page 14 ofprocesses. And finally, we speculate that regulation of these gene expressions it could be mediated through tRNAs IARS, GARS, EPRS and MARS. Regarding LT, we included ADG values as a covariate in the statistical model used to validate the expression differences by qPCR to avoid estimation biases. In this case, ADG was not significant, and a similar FC between microarray and qPCR results was found (r2 = 0.99; p < 0.0001). Thus, the treatment was also the main effect over gene expression of SF. Although we found differences in mRNA activity, it did not necessarily cause differences in metabolic processes. An increase in gene expression is not necessarily correlated with an increase in protein concentrations or enzyme activities. There are many processes between transcription and translation, including post-transcriptional, translational and protein degradation regulation, in controlling steady-state protein abundances [53]. Moreover, DNA microarray has been the technology of choice for transcriptome analysis in recent years. Nonetheless, array technology has several limitations which include: using microarray technology limits the researcher to detecting transcripts that correspond to existing genomic sequencing information; and background hybridization limits the accuracy of expression measurements, particularly for transcripts present in low abundance.and were approved by the Animal Welfare Committee of the Centro de Investigaci y Tecnolog agroalimentaria (CITA) (protocol number 2009-01_MJT). In all cases, euthanasia was performed by penetrating captive bolt followed by immediate exsanguination.Animals and sample collectionConclusion This study demonstrated the beneficial effects of vitamin E supplementation during fattening period in lambs by increasing -tocopherol content in the LT muscle and diminishing drastically the lipid oxidation of the meat. We observed a tissue-specific response to vitamin E supplementation. The gene expression profiles for VE and CON treatments were different in both LT and SF. Vitamin E supplementation had a dramatic effect on subcutaneous fat gene expression, showing a general up-regulation of genes, compared to CON treatment. Our study enabled us to identify novel genes (for example, IGF1R, ACAT1, ABCC4, ACACB, SOD3, and IER3) and metabolic pathways related to vitamin E metabolism that might be impli.