##plugins.themes.academic_pro.article.main##
Abstract
Plants offer considerable potential as natural sources of raw materials for the development of functional foods. Durian seeds (Durio zibethinus L.), which are often underutilized and treated as waste, present a promising resource due to their content of bioactive compounds with potential health benefits. This study aimed to determine the total phenolic and alkaloid contents in durian seed extract. Quantitative analysis was conducted using UV-Vis spectrophotometry. The results showed that the extract contained 64.1 ± 4.1 mg GAE/g of total phenolics and 445.19 ± 0.106 ppm of total alkaloids. These findings highlight the potential of durian seeds as a natural ingredient for developing functional food products. This research provides a scientific foundation for future studies, including in vitro and in vivo biological activity evaluations, to support the incorporation of durian seed extract into functional food formulations that contribute to improved health outcomes.
Keywords
##plugins.themes.academic_pro.article.details##
References
- Abu-odeh, A., Shehadeh, M., Suaifan, G. A. R. Y., Karameh, N., Rahman, D. A., & Kandil, Y. (2022). In Vitro and In Vivo Antidiabetic Activity, Phenolic Content, and Microscopical Characterization of Terfezia claveryi. Molecules, 2022(27), 1–14. https://doi.org/10.3390/molecules27154843
- Allo, I. S., Suryanto, E., & Koleangan, H. S. J. (2022). Aktivitas Antioksidan Fenolik Bebas dan Terikat dari Tepung Cangkang Pala (Myristica fragrans Houtt). Chemistry Progress, 15(2), 83–92. https://doi.org/10.35799/cp.15.2.2022.44496
- Amir, F., & Saleh, C. (2014). Uji Aktivitas Antioksidan Ekstrak Etanol Biji Buah Durian (Durio zibethinus Murr.) dengan Menggunakan Metode DPPH. Jurnal Kimia Mulawarman, 11(2), 86–87.
- Arwanto, V., Buschle-Diller, G., Mukti, Y. P., Dewi, A. D. R., Mumpuni, C., Purwanto, M. G. M., & Sukweenadhi, J. (2022). The state of plant-based food development and its prospects in the Indonesia market. Heliyon, 8(10), e11062. https://doi.org/10.1016/j.heliyon.2022.e11062
- Azka, N., Jayak Pratama, K., Riska Permata, B., Studi Farmasi, P., Ilmu Kesehatan, F., & Duta Bangsa Surakarta Abstract, U. (2023). Uji Aktivitas Antibakteri Ekstrak Metanol Dan Fraksi Biji Buah Durian Montong (Durio zibethinus Murr) Terhadap Bakteri Escherichia coli ATCC 25922. Jurnal Ilmiah Wahana Pendidikan, Desember, 9(25), 624–639. Retrieved from https://doi.org/10.5281/zenodo.10433798
- Bakrim, S., El Omari, N., El Hachlafi, N., Bakri, Y., Lee, L. H., & Bouyahya, A. (2022). Dietary Phenolic Compounds as Anticancer Natural Drugs: Recent Update on Molecular Mechanisms and Clinical Trials. Foods, 11(21), 1–33. https://doi.org/10.3390/foods11213323
- Boussouf, L., Boutennoune, H., Kebieche, M., Adjeroud, N., Al-Qaoud, K., & Madani, K. (2017). Anti-inflammatory, analgesic and antioxidant effects of phenolic compound from Algerian Mentha rotundifolia L. leaves on experimental animals. South African Journal of Botany, 113, 77–83. https://doi.org/10.1016/j.sajb.2017.07.003
- Chen, G. L., Chen, S. G., Chen, F., Xie, Y. Q., Han, M. Di, Luo, C. X., … Gao, Y. Q. (2016). Nutraceutical potential and antioxidant benefits of selected fruit seeds subjected to an in vitro digestion. Journal of Functional Foods, 20, 317–331. https://doi.org/10.1016/j.jff.2015.11.003
- Dewi, E. S., & Sari, D. A. (2024). Food Science and Technology Journal Phytochemical Tests and Antioxidant Activity Tests From Ethanol Extract Of Fig Leaves (Ficus carica Linn.) Green Yordan. Food Science and Technology Journal, 7(2), 151–160. https://doi.org/10.25139/fst.vi.8656
- El-Ramady, H., Hajdú, P., Törős, G., Badgar, K., Llana, X., Kiss, A., … Prokisch, J. (2022). Plant Nutrition for Human Health: A Pictorial Review on Plant Bioactive Compounds for Sustainable Agriculture. Sustainability (Switzerland), 14(14). https://doi.org/10.3390/su14148329
- Fauziyah, N., Widyasanti, A., & Sutresna, Y. (2022). Kajian Pengaruh Konsentrasi Etanol Terhadap Karakteristik Oleoresin Ampas Jahe Merah (Zingiber officinale Roscoe) Limbah Penyulingan. Teknotan, 16(3), 169. https://doi.org/10.24198/jt.vol16n3.6
- Frumuzachi, O., Flanagan, A., Rohn, S., & Mocan, A. (2025). The dichotomy between functional and functionalized foods – A critical characterization of concepts. Food Research International, 208(March). https://doi.org/10.1016/j.foodres.2025.116173
- Gan, J., Feng, Y., He, Z., Li, X., & Zhang, H. (2017). Correlations between Antioxidant Activity and Alkaloids and Phenols of Maca (Lepidium meyenii). Journal of Food Quality, 2017. https://doi.org/10.1155/2017/3185945
- Hidayah, N., Hisan, A. K., Solikin, A., Irawati, I., & Mustikaningtyas, D. (2016). Uji Efektivitas Ekstrak Sargassum muticum Sebagai Alternatif Obat Bisul Akibat Aktivitas Staphylococcus aureus. Journal of Creativity Student, 1(2). https://doi.org/10.15294/jcs.v1i2.7794
- Ho, L. H., & Bhat, R. (2015). Exploring the Potential Nutraceutical Values of Durian (Durio zibethinus L.) - An Exotic Tropical Fruit. Food Chemistry, 168, 80–89. https://doi.org/10.1016/j.foodchem.2014.07.020
- Indriyah, S. N., Permatasari, D. A. I., & Pratama, K. J. (2023). Penetapan Kadar Fenolik serta Uji Aktivitas Antioksidan Ekstrak dan Fraksi Batang Bajakah Kalalawit (Uncaria gambir Roxb) dengan Metode FRAP. Usada Nusantara: Jurnal Kesehatan Tradisional, 1(2), 147–158. https://doi.org/10.47861/usd.v1i2.347
- Irwandi, Sartika, D., & Putra, E. D. (2022). Efek Penyembuhan Luka Eksisi pada Tikus Putih Jantan (Rattus norvegicus) dengan Ekstrak Etanol Biji Buah Durian (Durio zibethinus L.) Selama 10 Hari. Jurnal Katalisator, 7(1), 90–101.
- Ketsa, S. (2018). Durian—Durio zibethinus. In Exotic Fruits Reference Guide (pp. 169–180). Bangkok: Elsevier Inc. https://doi.org/10.1016/B978-0-12-803138-4.00022-8
- Khaksar, G., Kasemcholathan, S., & Sirikantaramas, S. (2024). Durian (Durio zibethinus L.): Nutritional Composition, Pharmacological Implications, Value-Added Products, and Omics-Based Investigations. Horticulturae, 10(4), 1–23. https://doi.org/10.3390/horticulturae10040342
- Kumalaningrum, A. N., & Lestari, W. W. (2024). Effect of Alcohol Concentration in Soxhlet Extraction of Bioactive Compounds from Pineapple Peel as an Antioxidant for Cooking Oil. Food Science and Technology Journal, 7(2), 84–94.
- Kurniawan, H., Nurbaeti, S. N., Ih, H., Nugraha, F., & Fajriaty, I. (2024). Profil kadar metabolit sekunder : Steroid , alkaloid , dan tanin ekstrak daun bintangur (Calophyllum soulattri). Sasambo Kournal of Pharmacy, 5(2), 83–90.
- Kurniawati, A. D. (2023). Pengembangan Produk Pangan: Rancangan Penelitian dan Aplikasinya. Malang: UB Press.
- Ling, J. K. U., Lim, S. H., Khin, Y. Wi., Antipina, M. N., & Ng, S. B. (2025). Valorization of Macronutrients of Fruit Seeds and Their Food Applications: A Review. Food Reviews International, 1–22. https://doi.org/10.1080/87559129.2025.2486270
- Liu, W., Cui, X., Zhong, Y., Ma, R., Liu, B., & Xia, Y. (2023). Phenolic metabolites as therapeutic in inflammation and neoplasms: Molecular pathways explaining their efficacy. Pharmacological Research, 193(June), 106812. https://doi.org/10.1016/j.phrs.2023.106812
- Maharani, L., Zuhro, F., & Mahbubah, F. S. (2024). Test of Flavonoid Activity of Durian (Durio Zibethinus) Seeds and Skins as Natural Insecticides in Controlling Mealybug (Pseudococcidae). BIOEDUKASI: Jurnal Biologi Dan Pembelajarannya, 22(1), 152–156. https://doi.org/10.19184/bioedu.v22i1.44608
- Mahardani, O. T., & Yuanita, L. (2021). Efek Metode Pengolahan dan Penyimpanan Terhadap Kadar Senyawa Fenolik dan Aktivitas Antioksidan. Unesa Journal of Chemistry, 10(1), 64–78. https://doi.org/10.26740/ujc.v10n1.p64-78
- Maheshwari, N., & Sharma, M. C. (2023). Anticancer Properties of Some Selected Plant Phenolic Compounds: Future Leads for Therapeutic Development. Journal of Herbal Medicine, 42(October 2022), 100801. https://doi.org/10.1016/j.hermed.2023.100801
- Masitah, M., Pribadi, T., Pratama, M. I., Harrist, R. F., Sari, P. A., Dianita, F., & Setiawan, V. K. (2023). Analisis Kandungan Metabolik Sekunder pada Daun Kenikir (Cosmos caudatus Kunth.) dengan Pelarut Metanol, Etanol, dan Etil Asetat. BIOEDUKASI (Jurnal Pendidikan Biologi), 14(2), 266. https://doi.org/10.24127/bioedukasi.v14i2.7805
- Muhammad, I., Rahman, N., Gul-E-nayab, Nishan, U., & Shah, M. (2021). Antidiabetic activities of alkaloids isolated from medicinal plants. Brazilian Journal of Pharmaceutical Sciences, 57, 1–14. https://doi.org/10.1590/s2175-97902020000419130
- Mungmai, L., Kanokwattananon, C., Thakang, S., Nakkrathok, A., Srisuksomwong, P., & Tanamatayarat, P. (2023). Physicochemical Properties, Antioxidant and Anti-Tyrosinase Activities of Durio zibethinus Murray and Value Added for Cosmetic Product Formulation. Cosmetics, 10(3), 1–13. https://doi.org/10.3390/cosmetics10030087
- Mustofa, M. Z., Asmoro, N. W., & Handayani, C. B. (2024). Karakteristik Fisik dan Kimia Tepung Biji Durian (Durio zibethinus Murr.) dengan Perendaman Natrium Metabisulfit. Journal of Food and Agricultural Product, 4(2), 97–107.
- Nugerahani, I., Suteja, A. M., Widharna, R. M., & Marsono, Y. (2017). In vivo evaluation of monascus-fermented durian seed for antidiabetic and antihypercholesterol agent. Food Research, 1(3), 83–88. https://doi.org/10.26656/fr.2017.3.023
- Oluwafemi, A. B., Onuwabhagbe, O. G., Abiodun, O. P., Adefunke, A. O., Oladimeji, A. O., & Kola, O. J. (2022). Nutraceuticals: role in metabolic disease, prevention and treatment. Noticias Mundiales de Ciencias Naturales, 42(January), 1–27. Retrieved from www.worldnewsnaturalsciences.com
- Patel, K., Panchal, N., & Ingle, P. (2019). Review of Extraction Techniques Extraction Methods: Microwave, Ultrasonic, Pressurized Fluid, Soxhlet Extraction, Etc. International Journal of Advanced Research in Chemical Science, 6(3), 6–21. https://doi.org/10.20431/2349-0403.0603002
- Patel, S., & Rauf, A. (2017). Edible seeds from Cucurbitaceae family as potential functional foods: Immense promises, few concerns. Biomedicine and Pharmacotherapy, 91, 330–337. https://doi.org/10.1016/j.biopha.2017.04.090
- Permatasari, N. D., Witoyo, J. E., Masruri, M., Yuwono, S. S., & Widjanarko, S. B. (2021). Nutritional and Structural Properties of Durian Seed (Durio Zibenthinus Murr.) Flour Originated from West Kalimantan, Indonesia. IOP Conference Series: Earth and Environmental Science, 1012(1), 1–8. https://doi.org/10.1088/1755-1315/1012/1/012038
- Prayitno, S. A., Kusnadi, J., & Murtini, E. S. (2018). Karakteristik (Total Flavonoid, Total Fenol, Aktivitas Antioksidan) Ekstrak Serbuk Daun Sirih Merah (Piper crocatum Ruz & Pav.). Food Science and Technology Journal, 1(2), 26–34.
- Puri, V., Nagpal, M., Singh, I., Singh, M., Dhingra, G. A., Huanbutta, K., … Sangnim, T. (2022). A Comprehensive Review on Nutraceuticals: Therapy Support and Formulation Challenges. Nutrients, 14(21). https://doi.org/10.3390/nu14214637
- Purwaningsih, I., Hardiyati, R., Zulhamdani, M., Laksani, C. S., & Rianto, Y. (2021). Current Status of Functional Foods Research and Development in Indonesia: Opportunities and Challenges. Jurnal Teknologi Dan Industri Pangan, 32(1), 83–91. https://doi.org/10.6066/jtip.2021.32.1.83
- Ramadhan, H., Muthia, R., Wahyunita, S., Forestryana, D., Soleha, S. M., & Lihimi, L. (2023). Comparison of Extraction Solvents Towards Anti-Propionibacterium acnes activity of Alphitonia incana (Roxb). Teijsm. & Binn. ex Kurz Leaves. Indonesian Journal of Pharmaceutical Science and Technology, 1(1). https://doi.org/10.24198/ijpst.v0i0.45897
- Renda, Y. K., Pote, L. L., & Nadut, A. (2023). Isolasi dan Karakterisasi Senyawa Alkaloid dari Kulit Batang Tumbuhan Halay (Alstonia spectabilis R. Br) Asal Desa Wee Rame Kabupaten Sumba Barat Daya. Jurnal Sains Dan Edukasi Sains, 6(1), 44–50. https://doi.org/10.24246/juses.v6i1p44-50
- Riaz, M., Khalid, R., Afzal, M., Anjum, F., Fatima, H., Zia, S., … Aslam, M. A. (2023). Phytobioactive compounds as therapeutic agents for human diseases: A review. Food Science and Nutrition, 11(6), 2500–2529. https://doi.org/10.1002/fsn3.3308
- Simorangkir, M., Silaban, S., & Roza, D. (2022). Anticholesterol activity of ethanol extract of Ranti Hitam (Solanum blumei Nees ex Blume) Leaves: In vivo and In silico study. Pharmacia, 69(2), 485–492. https://doi.org/10.3897/pharmacia.69.e84913
- Sirumapea, L., Indryasari, S., Darwis, D., & Hilma. (2021). Perbandingan Total Fenolik Ekstrak Etanol Hasil Metode Maserasi dan Sokletasi dari Daun Papeda (Sonneratia alba Smith.) Menggunakan Spektrofotometri UV-Vis. Molluca Journal of Chemistry Education (MJoCE), 11(2), 74–80. https://doi.org/10.30598/mjocevol11iss2pp74-80
- Sultana, H., Alakeel, K. A., Hassan, J., Mallick, S. R., Zakaria, M., Kayesh, E., … Alamri, S. (2025). Nutrients, bioactive compounds and antinutritional properties of marigold genotypes as promising functional food. Scientific Reports, 15(1), 1–19. https://doi.org/10.1038/s41598-025-88694-x
- Takó, M., Kerekes, E. B., Zambrano, C., Kotogán, A., Papp, T., Krisch, J., & Vágvölgyi, C. (2020). Plant phenolics and phenolic-enriched extracts as antimicrobial agents against food-contaminating microorganisms. Antioxidants, 9(2). https://doi.org/10.3390/antiox9020165
- Vázquez-Ruiz, Z., Toledo, E., Vitelli-Storelli, F., Goni, L., de la O, V., Bes-Rastrollo, M., & Martínez-González, M. Á. (2022). Effect of Dietary Phenolic Compounds on Incidence of Cardiovascular Disease in the SUN Project; 10 Years of Follow-Up. Antioxidants, 11(4), 1–16. https://doi.org/10.3390/antiox11040783
- Velázquez-Martínez, V., Valles-Rosales, D., Rodríguez-Uribe, L., Laguna-Camacho, J. R., López-Calderón, H. D., & Delgado, E. (2022). Effect of Different Extraction Methods and Geographical Origins on the Total Phenolic Yield, Composition, and Antimicrobial Activity of Sugarcane Bagasse Extracts. Frontiers in Nutrition, 9(February), 1–10. https://doi.org/10.3389/fnut.2022.834557
- Wiyatno, F. W., Ayu, S., Putri, M., Sinthia, N. H., Sukhufi, Y., Muharikah, N., … Agustina, E. (2024). Analisis Kadar Senyawa Bioaktif “Fenolik” pada Daun Jeruk Purut dengan Metode Ekstraksi Maserasi dan Uji Kuantitatif Menggunakan Spektrofotometer UV-Vis. Jurnal Studi Multidisipliner, 8(12), 256–263.