Main Article Content

Abstract

Red dragon fruit peel (Hylocereus polyrhizus) has a higher antioxidant content than red dragon fruit
flesh. However, the peel of red dragon fruit is still not widely utilized in manufacturing functional foods.
Fermented drink is one type of product that uses microbes to decompose nutrients contained in raw
materials therefore the nutritional value of the resulting product can be increased. This study aimed to
make fermented drink from red dragon fruit peel which was done spontaneously with the addition of
palm sugar as a source of nutrients for microbes. The method used was a complete randomized design
(CRD) with one factor: the concentration of palm sugar (10%, 20%, 30%, and 40%) and the weight of
red dragon fruit peel as much as 60%. The results showed that the best dragon fruit peel fermented
drink formulation was using 30% palm sugar which produced the highest total titratable acidity of
0.36±0.05% with a pH value of 3.39±0.03. Total lactic acid bacteria also had the highest value of
3.30x109±0.06 CFU/ml while the reducing sugar content was 4.47±0.015%. The highest reducing sugar
(5.04±0.065%) was obtained in the formulation that uses 20% of palm sugar as carbon sources

Keywords

Lactic acid bacteria; Reducing sugar; Red dragon fruit peel; Spontaneous fermentation; Total titratable acidity

Article Details

How to Cite
Kusumasari, F. C., Sasmita, I. R. A., Fadhila, P. T., Mardiyanto, M., & Kurniawati, E. (2024). Development of Spontaneous Fermented Drink from Red Dragon Fruit Peel (Hylocereus polyrhizus): Chemical and Microbiological Perspectives. Food Science and Technology Journal (Foodscitech), 105-116. https://doi.org/10.25139/fst.vi.9109

References

  1. Alagbe, E. E., Okoye, G. C., Amoo, T. E., Adekeye, B. T., Taiwo, O. S., Adeyemi, A. O., & Daniel, E. O. (2022). Spontaneous and controlled fermentation to improve nutritional value of Ikpakpa beans, Phaseolus vulgaris. Cogent Engineering, 9(1). https://doi.org/10.1080/23311916.2022.2066823
  2. AOAC. (1995). Official Methods of Analysis of Association of Official Analytical Chemist. AOAC International. In AOAC International.
  3. AOAC. (2000). Official Methods of Analysis of Association of Official Analytical Chemist. In AOAC International.
  4. Bangar, S. P., Suri, S., Trif, M., & Ozogul, F. (2022). Organic acids production from lactic acid bacteria: A preservation approach. Food Bioscience, 46, 101615. https://doi.org/10.1016/j.fbio.2022.101615
  5. BPS. (2024). Produksi Tanaman Buah-buahan, 2021-2023. Badan Pusat Statistik.
  6. Cahyanti, A. N., Nofiyanto, E., Sampurno, A., Sudjatinah, M., & Wibowo, C. H. (2022). Viability of Lactic Acid Bacteria and Its Sugar Metabolism Capability in Fermentation Using Local Salak Extract. The 1st International Conference on Agricultural, Nutraceutical, and Food Science (ICANFS) 2022.
  7. Canonico, L., Agarbati, A., Comitini, F., & Ciani, M. (2022). Assessment of Spontaneous Fermentation and Non-Saccharomyces Sequential Fermentation in Verdicchio Wine at Winery Scale. Beverages, 8(3), 49. https://doi.org/10.3390/beverages8030049
  8. Cichońska, P., Kostyra, E., Piotrowska, A., Ścibisz, I., Roszko, M., & Ziarno, M. (2024). Enhancing the sensory and nutritional properties of bean-based and lentil-based beverages through fermentation and germination. LWT, 199, 116140. https://doi.org/10.1016/j.lwt.2024.116140
  9. Dali, N., Chairunnas, A., Amalia, H. A. M., & Puspitasari, S. A. A. (2024). The Effect of Acidity Level (pH) and Palm Sugar Sucrose Levels on the Quality of Brown Sugar. METANA, 20(1), 14–26. https://doi.org/10.14710/metana.v20i1.57772
  10. Dimero, F. N., & Tepora, T. F. (2018). Processing and Development of Dragon Fruit Wine. International Journal of Environment, Agriculture and Biotechnology, 3(5), 1943–1947. https://doi.org/10.22161/ijeab/3.5.49
  11. Douradinho, R., Sica, P., Tonoli, F., Mattos, E., Oliveira, M., Pinto, A., Mota, L., Faria, T., Costa, V. F., Leite, G., Arthur, V., Coelho, S., & Baptista, A. (2023). Osmotic Stress Alleviation in Saccharomyces cerevisiae for High Ethanol Fermentations with Different Wort Substrates. Stresses, 3(4), 813–826. https://doi.org/10.3390/stresses3040055
  12. Fardiaz, S. (1993). Analisis Mikrobiologi Pangan. PT. Raja Grafindo Persada.
  13. Ferreira, A. M., & Mendes-Faia, A. (2020). The Role of Yeasts and Lactic Acid Bacteria on the Metabolism of Organic Acids during Winemaking. Foods, 9(9), 1231. https://doi.org/10.3390/foods9091231
  14. Fuso, A., Bancalari, E., Castellone, V., Caligiani, A., Gatti, M., & Bottari, B. (2023). Feeding Lactic Acid Bacteria with Different Sugars: Effect on Exopolysaccharides (EPS) Production and Their Molecular Characteristics. Foods, 12(1), 215. https://doi.org/10.3390/foods12010215
  15. Gong, X., Zou, S., Liu, W., Yu, Z., & Liu, X. (2023). Analysis of yeast diversity during spontaneous fermentation of red and white pitaya. International Food Research Journal, 30(3), 796–804. https://doi.org/10.47836/ifrj.30.3.21
  16. González-Garcinuño, Á., Tabernero, A., Sánchez-Álvarez, J. M., Galán, M. A., & Martin del Valle, E. M. (2017). Effect of bacteria type and sucrose concentration on levan yield and its molecular weight. Microbial Cell Factories, 16(1), 91. https://doi.org/10.1186/s12934-017-0703-z
  17. Huan, P. T., Hien, N. M., & Anh, N. H. T. (2020). Optimization of alcoholic fermentation of dragon fruit juice using response surface methodology. Food Research, 4(5), 1529–1536. https://doi.org/10.26656/fr.2017.4(5).125
  18. Keșa, A.-L., Pop, C. R., Mudura, E., Salanță, L. C., Pasqualone, A., Dărab, C., Burja-Udrea, C., Zhao, H., & Coldea, T. E. (2021). Strategies to Improve the Potential Functionality of Fruit-Based Fermented Beverages. Plants, 10(11), 2263. https://doi.org/10.3390/plants10112263
  19. Kim, D.-H., Lee, S.-B., Kim, D.-H., Lee, S.-B., & Park, H.-D. (2018). Fermentation characteristics of campbell early grape wine inoculated with indigenous Korean wine yeasts encapsulated in Ca-alginate beads after air-blast drying. Italian Journal of Food Science, 30, 535–552. https://doi.org/10.14674/IJFS-1118
  20. Kłosowski, G., & Czupryński, B. (2006). Kinetics of acetals and esters formation during alcoholic fermentation of various starchy raw materials with application of yeasts Saccharomyces cerevisiae. Journal of Food Engineering, 72(3), 242–246. https://doi.org/10.1016/j.jfoodeng.2004.12.001
  21. Lim, T. W., Choo, K. Y., Lim, R. L. H., Pui, L. P., Tan, C. P., & Ho, C. W. (2023). The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools. Heliyon, 9(11), e21940. https://doi.org/10.1016/j.heliyon.2023.e21940
  22. Liu, H., Bao, M.-L., Chen, H.-L., & Li, Q. (2017). Impact of Sucrose Addition on the Physiochemical Properties and Volatile Compounds of “Shuangyou” Red Wines. Journal of Food Quality, 2017, 1–11. https://doi.org/10.1155/2017/2926041
  23. Lu, Y., Sun, F., Wang, W., Liu, Y., Wang, J., Sun, J., Mu, J., & Gao, Z. (2020). Effects of spontaneous fermentation on the microorganisms diversity and volatile compounds during ‘Marselan’ from grape to wine. LWT, 134, 110193. https://doi.org/10.1016/j.lwt.2020.110193
  24. Maryani, Y., Rochmat, A., Khastini, R. O., Kurniawan, T., & Saraswati, I. (2021). Identification of Macro Elements (Sucrose, Glucose and Fructose) and Micro Elements (Metal Minerals) in the Products of Palm Sugar, Coconut Sugar and Sugar Cane. https://doi.org/10.2991/absr.k.210304.051
  25. Najri, M., Antara, N. S., & Wijaya, I. M. M. (2022). Pengaruh Penambahan Gula dan Lama Fermentasi terhadap Karakteristik Selulosa Bakterial dari Kulit Pisang Kepok (Musa paradisiaca L.). Jurnal Rekayasa Dan Manajemen Agroindustri , 10(2), 211–220. https://doi.org/10.24843/JRMA.2022.v10.i02.p09
  26. Ngoc, T., Thinh, P., Mui, D., Uyen, L., Ngan, N., Tran, N., Khang, P., Huy, L., Minh, T., & Trung, N. (2024). Influences of Fermentation Conditions on the Chemical Composition of Red Dragon Fruit (Hylocereus polyrhizus) Wine. Beverages, 10(3), 61. https://doi.org/10.3390/beverages10030061
  27. Nishikito, D. F., Borges, A. C. A., Laurindo, L. F., Otoboni, A. M. M. B., Direito, R., Goulart, R. de A., Nicolau, C. C. T., Fiorini, A. M. R., Sinatora, R. V., & Barbalho, S. M. (2023). Anti-Inflammatory, Antioxidant, and Other Health Effects of Dragon Fruit and Potential Delivery Systems for Its Bioactive Compounds. Pharmaceutics, 15(1), 159. https://doi.org/10.3390/pharmaceutics15010159
  28. Nurhafsah, N., Laboko, A. I., Gobel, D. G., Fitriawaty, Novitasari, E., Muazam, A., Andriani, I., & Rahmi, R. (2023). Effect of red dragon fruit (Hylocereus polyrhizus) peel and pulp on jelly candy chemical composition and acceptance. IOP Conference Series: Earth and Environmental Science, 1183(1), 012059. https://doi.org/10.1088/1755-1315/1183/1/012059
  29. Sabira, Q. A. N., & Suryani, T. (2023). Glucose Levels and Organoleptic Quality Probiotic Tepache of Pineapple Peel on Variation of Sugar and Fermentation Duration. Proceeding of International Conference on Biology Education, Natural Science, and Technology, 1(1).
  30. Saha, G., Mukhim, C., Chandni, S., Singh, P., & Chanu, N. B. (2024). Dragon Fruit Cultivation. Vigyan Varta, 5(4). https://www.researchgate.net/publication/380214567
  31. Shah, K., Chen, J., Chen, J., & Qin, Y. (2023). Pitaya Nutrition, Biology, and Biotechnology: A Review. International Journal of Molecular Sciences, 24(18), 13986. https://doi.org/10.3390/ijms241813986
  32. Sinamo, K. N., Ginting, S., & Pratama, S. (2022). Effect of sugar concentration and fermentation time on secang kombucha drink. IOP Conference Series: Earth and Environmental Science, 977(1), 012080. https://doi.org/10.1088/1755-1315/977/1/012080
  33. Sionek, B., Szydłowska, A., Küçükgöz, K., & Kołożyn-Krajewska, D. (2023). Traditional and New Microorganisms in Lactic Acid Fermentation of Food. Fermentation, 9(12), 1019. https://doi.org/10.3390/fermentation9121019
  34. Suharman, Sutakwa, A., & Nadia, L. S. (2021). Effects Of Sucrose Addition To Lactic Acid Concentrations And Lactic Acid Bacteria Population Of Butterfly Pea (Clitoria Ternatea L.) Yogurt. Journal of Physics: Conference Series, 1823(1), 012038. https://doi.org/10.1088/1742-6596/1823/1/012038
  35. Suryaningsih, S., Muslim, B., & Djali, M. (2021). The antioxidant activity of roselle and dragon fruit peel functional drink in free radical inhibition. Journal of Physics: Conference Series, 1836(1), 012069. https://doi.org/10.1088/1742-6596/1836/1/012069
  36. Timmermans, E., Bautil, A., Brijs, K., Scheirlinck, I., Van der Meulen, R., & Courtin, C. M. (2022). Sugar Levels Determine Fermentation Dynamics during Yeast Pastry Making and Its Impact on Dough and Product Characteristics. Foods, 11(10), 1388. https://doi.org/10.3390/foods11101388
  37. Wang, Y., Wu, J., Lv, M., Shao, Z., Hungwe, M., Wang, J., Bai, X., Xie, J., Wang, Y., & Geng, W. (2021). Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.612285
  38. Xie, J., & Gänzle, M. (2023). Microbiology of fermented soy foods in Asia: Can we learn lessons for production of plant cheese analogues? International Journal of Food Microbiology, 407, 110399. https://doi.org/10.1016/j.ijfoodmicro.2023.110399
  39. Zheng, H., Jiang, J., Huang, C., Wang, X., & Hu, P. (2024). Effect of sugar content on characteristic flavour formation of tomato sour soup fermented by Lacticaseibacillus casei H1 based on non-targeted metabolomics analysis. Food Chemistry: X, 21, 101116. https://doi.org/10.1016/j.fochx.2024.101116